ifferent Fatty Acid Composition Between ormal and Malignant Cell Lines 7 57

Twentyeight fatty aclds (C8:O to C24:ln-9) were measured by gas chromattography In four normal cell lines (C3H/lOT1/2, CCD18Co, CCD25SK and CCD 37Lu) an# seven cancer cell llnes ( M I , Caov-3, LS-180, PC3, SK-MEL-28, SK-MES-l and U-87 MG). Results show that C20:4 n-6, C24:0, C24:l n-9, Polyunsaturated fatty aclds (PUFA'S) and ratlos of C20:4 n-6 to C20:5 m3 and C16:O to C18:l n-9 and steatlc to olelc (SA/OA) were lower In the cancer cell llnes than In their normal courterparts. All cancer cell llnes had SA/OA ratlos lower than 0.7 while normal cell llnes had ratlos greater than 0.7 (p<0.05). In addition, the ratlos of total saturated fatty aclds (SFA) to PUFA'S and the concentration of C18:l rr 9, Cl8:2 n-6, C20:0, C20:2 n-6, C20:5 n-3 were higher In cancer cell llnes as compared to normal cell llnes. A positive cottelation was detected between C16:O and longer SFA'S (r=+0.511, p<0.05) In normal cell llnes whereas a negative correlation (r=0.608, ~ ~ 0 . 0 5 ) was obtained for malignant cell lines. Moreover, cancerous cell lines exhlb lted a particular desaturatlon defect and an abnormal lncorporatlon of C18:2 n-6 and C20-4 n-6.